منابع مشابه
Cell isolation and culture.
Cell isolation and culture are essential tools for the study of cell function. Isolated cells grown under controlled conditions can be manipulated and imaged at a level of resolution that is not possible in whole animals or even tissue explants. Recent advances have allowed for large-scale isolation and culture of primary C. elegans cells from both embryos and all four larval stages. Isolated c...
متن کاملIsolation and detection of Mycoplasma pneumoniae from cell culture by culture and PCR.
Background: Mycoplasma pneumoniae is one of the major species of mycoplasmas which could contaminate cell cultures. Identification of M. pneuminae is significant because Mycoplasmas could contaminate and have unsuitable effect on the cell cultures. Rapid detection of these contaminations is so important and it could be significant role in preventing and controlling of contamination in cell cul...
متن کاملIsolation in Cell Culture
The aetiology of inflammatory bowel disease is at present obscure, but there is evidence that transmissible agents such as bacteria or viruses may be associated with this condition.' 4 Chlamydia trachomatis agents are known to infect the rectum5 6 and lymphogranuloma venereum (LGV) serotypes can cause chronic inflammation with fistula formation similar to manifestations of Crohn's disease. Prev...
متن کاملMouse liver cell culture. I. Hepatocyte isolation.
A method for isolation of mouse liver cells by a two-step perfusion with calcium and magnesium-free Hanks' salt solution followed by a medium containing collagenase is described. Several variations of the commonly used procedure for rat liver cell isolation were quantitatively compared with respect to cell yield and viability. The optimal isolation technique involved perfusion through the hepat...
متن کاملIsolation and In vitro Culture of Pig Spermatogonial Stem Cell*
The present study identified the favorable conditions for isolation, enrichment and in vitro culture of highly purified, undifferentiated pig spermatogonial stem cell (SSC) lines that proliferate for long periods of time in culture. The colonies displayed morphology similar to miceSSC and were positive for markers of SSC (PGP9.5), proliferating germ cell (PigVASA), pre-meiotic germ cell (DAZL) ...
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ژورنال
عنوان ژورنال: WormBook
سال: 2013
ISSN: 1551-8507
DOI: 10.1895/wormbook.1.157.1